Method(s)
Gas Chromatography/Mass Spectrometry (GC/MS)
Suggested CPT Code(s)
80375
New York State Approval Status

Approved

Turnaround Time

7 days

Test Includes
1 Benzocaine Orabase; Oragel Gas Chromatography/Mass Spectrometry (GC/MS) mcg/mL 0.005 Anesthetic (Topical)

Test Also Known As
; Americaine®
Compliance Statement
This test was developed and its performance characteristics determined by NMS Labs. It has not been cleared or approved by the US Food and Drug Administration.
Sample Type
Plasma
Requested Volume
10 mL
Minimum Volume
4.8 mL
Special Handling
Step 1: Prepare esterase inhibitor solution (Neostigmine 2 mg/mL): Weigh 68 mg of Neostigmine Bromide (Sigma Chemical, St. Louis, MO) and transfer to a 25 mL volumetric flask. Dissolve and dilute to volume with deionized water, mix thoroughly. Store in an amber bottle at room temperature and discard nine (9) months after preparation. STEP 2: Prepare collection tubes to 3 mL plasma draw tubes containing Na2EDTA anti-coagulant, add 20 microliters of the 2 mg/mL Neostigmine solution without breaking the vacuum. This is accomplished by using a 25 microliter syringe and piercing the rubber top of the container. STEP 3: Collecting samples and storage: Draw blood into the prepared collection tubes, making efforts to draw at least 2.5 mL. Invert tube at least eight (8) times to mix with the EDTA and Neostigmine. Immediately centrifuge and carefully transfer, with plastic pipette, the supernatant plasma into a labeled plastic container. Keep refrigerated and freeze if stored for more than one week.
Sample Container
NMS Labs has no experimental or literature-based data regarding the choice of specific specimen collection containers for this test.

Additional Collection Instructions

Transport Temperature
Refrigerated
Light Protection
Not Required
Rejection Criteria
None

Rejection criteria pertain to clinical sample submissions only.

Stability
Room Temperature: Undetermined
Refrigerated: Undetermined
Frozen (-20 °C): Undetermined

The CPT Codes provided in this document are based on AMA Guidelines and are for informational purposes only. NMS Labs Does not assume responsibility for billing errors due to Reliance on the CPT Codes listed in this document.

*The information contained in this document represents database configurations, as they will appear on the effective date listed above.